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M9490072.TXT
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1994-09-03
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Document 0072
DOCN M9490072
TI Analysis of murine antibody responses to baculovirus-expressed human
immunodeficiency virus type 1 envelope glycoproteins.
DT 9411
AU Bristow RG; Douglas AR; Skehel JJ; Daniels RS; Virology Division,
National Institute for Medical Research, Mill; Hill, London, U.K.
SO J Gen Virol. 1994 Aug;75 ( Pt 8):2089-95. Unique Identifier : AIDSLINE
MED/94322004
AB An analysis of the humoral immunogenicity of a candidate AIDS vaccine
(VaxSyn) in a murine model system is presented. Sera taken from a panel
of mice immunized with the immunogen were analysed for their ability to
bind a panel of gp120-representing peptides and limited reactivity to
known sites of immunological interest was observed. Monoclonal
antibodies (MAbs) were characterized as binding to a more restricted
variety of regions on gp120 including C1, V2, V4 and the C terminus. A
tetrazolium-based cytocidicity assay was used and shown to be an
effective and objective method for the screening of human
immunodeficiency virus (HIV)-neutralizing activity in large numbers of
samples. None of the MAbs characterized in this study neutralize HIV-1
reference strains. The significance of these findings in view of
previous publications is discussed.
DE Animal Antibodies, Monoclonal/BIOSYNTHESIS Antigenic Determinants
AIDS Vaccines/*IMMUNOLOGY Baculoviridae/GENETICS HIV/*IMMUNOLOGY HIV
Antibodies/*BIOSYNTHESIS Mice Mice, Inbred BALB C Neutralization
Tests/METHODS Peptide Fragments/IMMUNOLOGY Recombinant
Proteins/IMMUNOLOGY Sialoglycoproteins/GENETICS/IMMUNOLOGY Support,
Non-U.S. Gov't Vaccines, Synthetic/*IMMUNOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).